Ming-Han Tsai蔡明翰副教授 - 國立陽明交通大學微生物及免疫學研究所

蔡明翰副教授

微生物及免疫學研究所副教授
專長領域:病毒學、生物科技、癌症生物學、免疫學、藥物篩選
辦公室位置: 生物醫學大樓(R411)
TEL (辦公室): +886-2-28267109
TEL (實驗室): +886-2-28267000 ext65626
E-mail: m.tsai@nycu.edu.tw
Website://wisewind1005.wixsite.com/tsaimh-ebv
ORCID: 0000-0001-5563-3057

學歷與經歷

Education and Experience

INSTITUTION AND LOCATION	DEGREE/ POSITION	FIELD OF RESEARCH
National Yang Ming Chiao Tung University, Taiwan	Assistant Professor 2018-	Virology, Biotechnology, and Cancer biology
German Cancer Research Center (DKFZ), Germany	Scientist 2015-17	Virology and Biotechnology
Ruprecht Karl University of Heidelberg, Germany	Ph.D. 2015	Virology and Oncology
National Health Research Institute, Taiwan	RA 2007-10	Virology, antiviral drug development
National Tsing-Hua University, Taiwan	M.S. 2006	Biotechnology and Immunology
National Tsing-Hua University, Taiwan	B.S. 2004	Life Science

獲獎

2021-2023 特殊優秀教研人員獎勵
2021國立陽明交通大學優良導師
2020,2021 Li-Yang Shen memorial award 沈力揚先生紀念獎
2020國立陽明交通大學醫學系良師益友獎
2018-2023 MOST Young Scholar Fellowships-Columbus program 科技部年輕學者養成計畫-哥倫布計畫學者

論文

https://wisewind1005.wixsite.com/tsaimh-ebv/publications

ORCID: 0000-0001-5563-3057

Hsieh, H.Y., Chang, R., Huang, Y.Y., Juan, P.H., Tahara, H., Lee, K.Y., Vo, D.N.K., Tsai, M.H., Wei, P.K., Sheen, H.J., Fan, Y.J. (2022, Jan). Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection. Biosensors and Bioelectronics, 10.1016/j.bios.2021.113672.
Li, Zhe., Baccianti, F., Delecluse, S., Tsai, M.H., Shumilov, A., Cheng, X., Ma, S., Hoffmann, I., Poirey, R., Delecluse, H.J. (2021, Oct). The Epstein-Barr virus non-coding RNA EBER2 transactivates the UCHL1 deubiquitinase to accelerate cell growth. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
Delecluse, S., Poirey, R., Zeier, M., Schnitzler, P., Behrends, U., Tsai, M.H., Delecluse, H.J. (corresponding). 2020. Identification and cloning of a new western Epstein-Barr virus strain that replicates efficiently in primary B cells. J. Virol. doi: 10.1128/JVI.01918-19.
Delecluse, S., Yu, J., Bernhardt, K., Haar, J., Poirey, R., Tsai, M.H., Kiblawi, R., Kopp-Schneider, A., Schnitzler, P., Zeier, M., Dreger, P., Wuchter, P., Bulut, OC., Behrends, U., Delecluse, H.J.. 2019. Spontaneous lymphoblastoid cell lines from patients with Epstein-Barr virus infection show highly variable proliferation characteristics that correlate with the expression levels of viral microRNAs. PloS One. doi: 10.1371/journal.pone.0222847
Li, Zhe.*, Tsai, M.H.* (*co-first), Shumilov, A., Baccianti, F., Tsao, S.W., Poirey, R., Delecluse, H.J., 2019. A non-coding RNA from a carcinogenic virus induces an inflammatory response to promote virus production in infected cells. Nature Microbiology. doi: 10.1038/s41564-019-0546-y
Tian, J.N., Yang, C.C., Chuang, C.K., Tsai, M.H., Wu, R.H., Chen, C.T., Yueh, A. 2019. A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication. Viruses. doi: 10.3390/v11090787.
Delecluse, S., Tsai, M.H., Shumilov, A., Bencun, M., Arrow, S., Beshirova, A., Cottignies-Calamarte, A., Lasitschka, F., Bulut, OC., Münz, C., Zeier, M., Behrends, U., Delecluse, H.J.. 2019. Epstein-Barr virus induces expression of the LPAM-1 integrin in B cells in vitro and in vivo. J. Virol. 19;93(5). pii: e01618-18.
van Zyl, D.G., Tsai, M.H., Shumilov, A., Schneidt, V., Poirey, R., Schlehe, B., Fluhr, H., Mautner, J., Delecluse, H.J.. 2018. Immunogenic particles with a broad antigenic spectrum stimulate cytolytic T cells and offer increased protection against EBV infection ex vivo and in mice. PLoS Pathog 14(12):e1007464
Bencun, M., Klinke, O., Hotz-Wagenblatt, A., Klaus, S., Tsai, M.H., Poirey, R., Delecluse, H.J. 2018. Translational profiling of B cells infected with the Epstein-Barr virus reveals 5’ leader ribosome recruitment through upstream opening reading frames. Nucleic Acids Res. 6;46(6):2802-2819.
Gupta, S., Ylä-Anttila, P., Callegari, S., Tsai, M.H., Delecluse, H.J., Masucci, M.G. 2018. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathog 14(1):e1006852.
Bilger, A., Plowshay, J., Ma, S.D., Nawandar, D., Barlow, E.A., Romero-Masters, J.C., Bristol, J.A., Li, Z., Tsai, M.H., Delecluse, H.J., Kenny, S.C. 2017. Leflunomide/teriflunomide inhibit EBV-induced lymphoproliferative disease and lytic replication. Oncotarget. 8: 44266-33280.
Wu, R.H., Tsai, M.H., Tsai, K.N., Tian, J.N., Wu, J.S., Wu, S.Y., Chern, J.H., Chen, C.H., and Yueh, A. 2017. Mutagenesis of Dengue Virus Protein NS2A Revealed a Novel Domain Responsible for Virus-Induced Cytopathic Effect and Interactions Between NS2A and NS2B Transmembrane Segments. J Virol. doi: 10.1128/jvi.01836-16 (Epub ahead of print).
Shumilov, A.*, Tsai, M.H.* (*co-first), Schlosser, Y., Kratz, A.S., Bernhardt, K., Fink, S., Mizani, T., Lin, X., Jauch, A., Mautner, J., et al. 2017. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nature Communications. doi:10.1038/ncomms14257.
Ma, S.D.*, Tsai, M.H.* (*co-first), Romero-Masters, J., Ranheim, E.A., Bristol, J.A., Delecluse, H.J., and Kenney, S.C. 2017. Epstein-Barr virus (EBV) can induce B cell lymphomas in a cord blood-humanized mouse model in the absence of LMP2A and LMP1 expression. J Virol. doi: 10.1128/jvi.01928-16.
Fink, S., Tsai, M.H., Schnitzler, P., Zeier, M., Dreger, P., Wuchter, P., Bulut, O.C., Behrends, U., and Delecluse, H.J. 2017. The Epstein-Barr virus DNA load in the peripheral blood of transplant recipients does not accurately reflect the burden of infected cells. Transpl Int 30:57-67.
Tsai, M.H., Lin, X., Shumilov, A., Bernhardt, K., Feederle, R., Poirey, R., Kopp-Schneider, A., Pereira, B., Almeida, R., and Delecluse, H.J. 2016. The biological properties of different Epstein-Barr virus strains explain their association with various types of cancers. Oncotarget. 10.18632/oncotarget.14380
Bernhardt, K., Haar, J., Tsai, M.H., Poirey, R., Feederle, R., and Delecluse, H.J. 2016. A Viral microRNA Cluster Regulates the Expression of PTEN, p27 and of a bcl-2 Homolog. PLoS Pathog 12:e1005405.
Wu, R.H., Tsai, M.H., Chao, D.Y., and Yueh, A. 2015. Scanning mutagenesis studies reveal a potential intramolecular interaction within the C-terminal half of dengue virus NS2A involved in viral RNA replication and virus assembly and secretion. J Virol 89:4281-4295.
Lin, XC, Tsai, M.H.* (*co-first author), Shumilov, A., Poirey, R., Bannert, H., Middeldorp, J.M., Feederle, R., and Delecluse, H.J. 2015. The Epstein-Barr Virus BART miRNA Cluster of the M81 Strain Modulates Multiple Functions in Primary B Cells. PLoS Pathog 11:e1005344.
Hou, M.S., Huang, S.T., Tsai, M.H., Yen, C.C., Lai, Y.G., Liou, Y.H., Lin, C.K., and Liao, N.S. 2015. The interleukin-15 system suppresses T cell-mediated autoimmunity by regulating negative selection and nT(H)17 cell homeostasis in the thymus. J Autoimmun 56:118-129.
Feederle, R., Klinke, O., Kutikhin, A., Poirey, R., Tsai, M.H., and Delecluse, H.J. 2015. Epstein-Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types. Curr Top Microbiol Immunol 390:119-148.
Pu, S.Y., Wu, R.H., Tsai, M.H., Yang, C.C., Chang, C.M., and Yueh, A. 2014. A novel approach to propagate flavivirus infectious cDNA clones in bacteria by introducing tandem repeat sequences upstream of virus genome. J Gen Virol 95:1493-1503.
Antsiferova, O., Muller, A., Ramer, P.C., Chijioke, O., Chatterjee, B., Raykova, A., Planas, R., Sospedra, M., Shumilov, A., Tsai, M.H., et al. 2014. Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. PLoS Pathog 10:e1004333.
Yang, C.C., Tsai, M.H., Hu, H.S., Pu, S.Y., Wu, R.H., Wu, S.H., Lin, H.M., Song, J.S., Chao, Y.S., and Yueh, A. 2013. Characterization of an efficient dengue virus replicon for development of assays of discovery of small molecules against dengue virus. Antiviral Res 98:228-241.
Tsai, M.H., Raykova, A., Klinke, O., Bernhardt, K., Gartner, K., Leung, C.S., Geletneky, K., Sertel, S., Munz, C., Feederle, R., et al. 2013. Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas. Cell Rep 5:458-470. (Spotlight article in the review Frappier, L. 2013. A cancer-associated Epstein-Barr virus variant with epithelial tropism. Trends Microbiol 21:616-617)
Pu, S.Y., Wu, R.H., Yang, C.C., Jao, T.M., Tsai, M.H., Wang, J.C., Lin, H.M., Chao, Y.S., and Yueh, A. 2011. Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes. J Virol 85:2927-2941.
Lai, Y.G., Hou, M.S., Hsu, Y.W., Chang, C.L., Liou, Y.H., Tsai, M.H., Lee, F., and Liao, N.S. 2008. IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine. J Immunol 180:3757-3765.

實驗室介紹

Current Research

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research

簡介: 我們對於癌症病毒，特別是EB病毒(EBV,台灣常稱鼻咽癌病毒)如何導致人類上皮細胞癌深感興趣。除了鼻咽癌，一種和EBV絕對相關的癌症，目前也發現EBV在胃癌、肺癌、乳癌、大腸癌、淋巴癌、胸腺癌等各類癌症，甚至多種自體免疫疾病，都可大量發現EBV感染的證據。很可惜EBV到底如何導致這些疾病仍不清楚。

我們實驗室具備全世界對此病毒研究之最先進技術，當前我們將主力放在此病毒對胃癌造成之機制。我們也同時研究一些新發現由此病毒導致之癌症種類及疾病。

同時，我們也嘗試改造這些致癌病毒使之為我們所用。我們有專門技術可以去改造大體積之DNA病毒並且成功將之送到宿主細胞內。期待我們能將這些”壞壞”的病毒轉化變成人類有用的工具。竭誠歡迎您加入我們並用研究病毒來改變這個世界!

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research

實驗室成員

-洪若寧 (Joning)

-蔡若翎 (Rolling)

-周壬渼 (MeiMei)

-Di Ngoc Kha Vo (Zoe)

-Ha Phan Thanh Ho (Hani)

-林榆涵 (Irene)

-邱雅彙 (Vicky)

-吳思賢 (Sam)

-陳羿妘 (Yiyun)

-All Members and Alumni please visit here https://wisewind1005.wixsite.com/tsaimh-ebv/people

蔡明翰 副教授

學歷與經歷

Education and Experience

INSTITUTION AND LOCATION

DEGREE/ POSITION

FIELD OF RESEARCH

National Yang Ming Chiao Tung University, Taiwan

Assistant Professor

2018-

Virology, Biotechnology, and Cancer biology

German Cancer Research Center (DKFZ), Germany

Scientist

2015-17

Virology and Biotechnology

Ruprecht Karl University of Heidelberg, Germany

Ph.D.

2015

Virology and Oncology

National Health Research Institute, Taiwan

RA

2007-10

Virology, antiviral drug development

National Tsing-Hua University, Taiwan

M.S.

2006

Biotechnology and Immunology

National Tsing-Hua University, Taiwan

B.S.

2004

Life Science

獲獎

2021-2023 特殊優秀教研人員獎勵

2021國立陽明交通大學優良導師

2020,2021 Li-Yang Shen memorial award 沈力揚先生紀念獎

2020國立陽明交通大學醫學系良師益友獎

2018-2023 MOST Young Scholar Fellowships-Columbus program 科技部年輕學者養成計畫-哥倫布計畫學者

論文

https://wisewind1005.wixsite.com/tsaimh-ebv/publications

ORCID: 0000-0001-5563-3057

Delecluse, S., Poirey, R., Zeier, M., Schnitzler, P., Behrends, U., Tsai, M.H., Delecluse, H.J. (corresponding). 2020. Identification and cloning of a new western Epstein-Barr virus strain that replicates efficiently in primary B cells. J. Virol. doi: 10.1128/JVI.01918-19.

Li, Zhe.*, Tsai, M.H.* (*co-first), Shumilov, A., Baccianti, F., Tsao, S.W., Poirey, R., Delecluse, H.J., 2019. A non-coding RNA from a carcinogenic virus induces an inflammatory response to promote virus production in infected cells. Nature Microbiology. doi: 10.1038/s41564-019-0546-y

Tian, J.N., Yang, C.C., Chuang, C.K., Tsai, M.H., Wu, R.H., Chen, C.T., Yueh, A. 2019. A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication. Viruses. doi: 10.3390/v11090787.

Bencun, M., Klinke, O., Hotz-Wagenblatt, A., Klaus, S., Tsai, M.H., Poirey, R., Delecluse, H.J. 2018. Translational profiling of B cells infected with the Epstein-Barr virus reveals 5’ leader ribosome recruitment through upstream opening reading frames. Nucleic Acids Res. 6;46(6):2802-2819.

Gupta, S., Ylä-Anttila, P., Callegari, S., Tsai, M.H., Delecluse, H.J., Masucci, M.G. 2018. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathog 14(1):e1006852.

Bilger, A., Plowshay, J., Ma, S.D., Nawandar, D., Barlow, E.A., Romero-Masters, J.C., Bristol, J.A., Li, Z., Tsai, M.H., Delecluse, H.J., Kenny, S.C. 2017. Leflunomide/teriflunomide inhibit EBV-induced lymphoproliferative disease and lytic replication. Oncotarget. 8: 44266-33280.

Shumilov, A.*, Tsai, M.H.* (*co-first), Schlosser, Y., Kratz, A.S., Bernhardt, K., Fink, S., Mizani, T., Lin, X., Jauch, A., Mautner, J., et al. 2017. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nature Communications. doi:10.1038/ncomms14257.

Ma, S.D.*, Tsai, M.H.* (*co-first), Romero-Masters, J., Ranheim, E.A., Bristol, J.A., Delecluse, H.J., and Kenney, S.C. 2017. Epstein-Barr virus (EBV) can induce B cell lymphomas in a cord blood-humanized mouse model in the absence of LMP2A and LMP1 expression. J Virol. doi: 10.1128/jvi.01928-16.

Fink, S., Tsai, M.H., Schnitzler, P., Zeier, M., Dreger, P., Wuchter, P., Bulut, O.C., Behrends, U., and Delecluse, H.J. 2017. The Epstein-Barr virus DNA load in the peripheral blood of transplant recipients does not accurately reflect the burden of infected cells. Transpl Int 30:57-67.

Bernhardt, K., Haar, J., Tsai, M.H., Poirey, R., Feederle, R., and Delecluse, H.J. 2016. A Viral microRNA Cluster Regulates the Expression of PTEN, p27 and of a bcl-2 Homolog. PLoS Pathog 12:e1005405.

Wu, R.H., Tsai, M.H., Chao, D.Y., and Yueh, A. 2015. Scanning mutagenesis studies reveal a potential intramolecular interaction within the C-terminal half of dengue virus NS2A involved in viral RNA replication and virus assembly and secretion. J Virol 89:4281-4295.

Lin, XC, Tsai, M.H.* (*co-first author), Shumilov, A., Poirey, R., Bannert, H., Middeldorp, J.M., Feederle, R., and Delecluse, H.J. 2015. The Epstein-Barr Virus BART miRNA Cluster of the M81 Strain Modulates Multiple Functions in Primary B Cells. PLoS Pathog 11:e1005344.

Hou, M.S., Huang, S.T., Tsai, M.H., Yen, C.C., Lai, Y.G., Liou, Y.H., Lin, C.K., and Liao, N.S. 2015. The interleukin-15 system suppresses T cell-mediated autoimmunity by regulating negative selection and nT(H)17 cell homeostasis in the thymus. J Autoimmun 56:118-129.

Feederle, R., Klinke, O., Kutikhin, A., Poirey, R., Tsai, M.H., and Delecluse, H.J. 2015. Epstein-Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types. Curr Top Microbiol Immunol 390:119-148.

Pu, S.Y., Wu, R.H., Tsai, M.H., Yang, C.C., Chang, C.M., and Yueh, A. 2014. A novel approach to propagate flavivirus infectious cDNA clones in bacteria by introducing tandem repeat sequences upstream of virus genome. J Gen Virol 95:1493-1503.

Antsiferova, O., Muller, A., Ramer, P.C., Chijioke, O., Chatterjee, B., Raykova, A., Planas, R., Sospedra, M., Shumilov, A., Tsai, M.H., et al. 2014. Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. PLoS Pathog 10:e1004333.

Yang, C.C., Tsai, M.H., Hu, H.S., Pu, S.Y., Wu, R.H., Wu, S.H., Lin, H.M., Song, J.S., Chao, Y.S., and Yueh, A. 2013. Characterization of an efficient dengue virus replicon for development of assays of discovery of small molecules against dengue virus. Antiviral Res 98:228-241.

Pu, S.Y., Wu, R.H., Yang, C.C., Jao, T.M., Tsai, M.H., Wang, J.C., Lin, H.M., Chao, Y.S., and Yueh, A. 2011. Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes. J Virol 85:2927-2941.

Lai, Y.G., Hou, M.S., Hsu, Y.W., Chang, C.L., Liou, Y.H., Tsai, M.H., Lee, F., and Liao, N.S. 2008. IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine. J Immunol 180:3757-3765.

實驗室介紹

Current Research

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research

我們實驗室具備全世界對此病毒研究之最先進技術，當前我們將主力放在此病毒對胃癌造成之機制。我們也同時研究一些新發現由此病毒導致之癌症種類及疾病。

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research

實驗室成員

-洪若寧 (Joning)

-蔡若翎 (Rolling)

-周壬渼 (MeiMei)

-Di Ngoc Kha Vo (Zoe)

-Ha Phan Thanh Ho (Hani)

-林榆涵 (Irene)

-邱雅彙 (Vicky)

-吳思賢 (Sam)

-陳羿妘 (Yiyun)

-All Members and Alumni please visit here https://wisewind1005.wixsite.com/tsaimh-ebv/people

實驗室照片

2022雙寶滿百日轟趴

2021換牌前留念

2021關渡定向越野

2020開山大師兄大師姊畢業趴+特製德國風畢業帽

蔡明翰副教授

Li, Zhe., Tsai, M.H. (*co-first), Shumilov, A., Baccianti, F., Tsao, S.W., Poirey, R., Delecluse, H.J., 2019. A non-coding RNA from a carcinogenic virus induces an inflammatory response to promote virus production in infected cells. Nature Microbiology. doi: 10.1038/s41564-019-0546-y

Shumilov, A., Tsai, M.H. (*co-first), Schlosser, Y., Kratz, A.S., Bernhardt, K., Fink, S., Mizani, T., Lin, X., Jauch, A., Mautner, J., et al. 2017. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nature Communications. doi:10.1038/ncomms14257.

Ma, S.D., Tsai, M.H. (*co-first), Romero-Masters, J., Ranheim, E.A., Bristol, J.A., Delecluse, H.J., and Kenney, S.C. 2017. Epstein-Barr virus (EBV) can induce B cell lymphomas in a cord blood-humanized mouse model in the absence of LMP2A and LMP1 expression. J Virol. doi: 10.1128/jvi.01928-16.