蔡明翰副教授
Ming-Han Tsai

1. 國立陽明交通大學優良教學獎,Sep 2025.
2. 國科會吳大猷先生紀念獎, 2023
3. MOST Young Scholar Fellowship, Taiwan (科技部年輕學者養成計畫), 2018-2023
4. 美國、韓國、俄羅斯等國際發明獎金牌, 2022, 2023
5. 財團法人沈力揚教授醫學教育獎學紀念基金會/講師及研究與進修獎助金, 2020,2021
6. 國立陽明交通大學110 年度優良導師, Sep 2021
7. 國立陽明大學109 年度良師益友導師, Sep 2020
   其他資訊 https://wisewind1005.wixsite.com/tsaimh-ebv/pi

論文著作 :

Website: https://wisewind1005.wixsite.com/tsaimh-ebv/publications

ORCID: https://orcid.org/0000-0001-5563-3057

A1. 2018-now (annotated by JCR 2025 https://jcr.clarivate.com/jcr/home)

1. Ren-Jie Weng#, Li-Syuan Wu#, Darwin Kurniawan, Ching-Tzu Chang, Ming-Han Tsai*, Wei-Hung Chiang*(2025, Oct). Plasma-Guided Green Synthesis of Crystalline Graphene Quantum Dots for Broad-Spectrum Antiviral Applications. Chemical Engineering Journal (3%: 3/83 ENGINEERING, ENVIRONMENTAL, IF 13.2) (Accepted, *corresponding).
2. Di Ngoc Kha Vo, Ha Phan Thanh Ho, Li-Syuan Wu, Yi-Yun Chen, Hang Thi Viet Trinh, Tung-Yi Lin, Yat-Yuen Lim, Keng-Chang Tsai, Ming-Han Tsai*(2025, Mar). Broad-spectrum antiviral activity of Ganoderma microsporum immunomodulatory protein: Targeting glycoprotein gB to inhibit EBV and HSV-1 infections via viral fusion blockage. International Journal of Biological Macromolecules, (5.9%: 6/94 Polymer Science, IF 8.2) Volume 307, Part 3, May 2025, 142179 (*corresponding).
3. Yi Chiung Hsu, Ming-Han Tsai, Guani Wu, Chien Liang Liu, Yuan Ching Chang, Hung Bun Lam, Pei Yu Su, Chun Fan Lung, Po Sheng Yang.(2024, Mar). Role of Epstein-Barr Virus in Breast Cancer: Correlation with Clinical Outcome and Survival Analysis. Journal of Cancer, 2024 Mar 4;15(8):2403–2411.
4. Wing Chung Tang, Sai Wah Tsao, Gareth E Jones, Xiong Liu, Ming-Han Tsai, Henri-Jacques Delecluse , Wei Dai, Chanping You, Jun Zhang, Shaina Chor Mei Huang, Manton Man-Hon Leung, Tengfei Liu, Yick Pang Ching, Honglin Chen, Kwok Wai Lo, Xin Li, Chi Man Tsang (2023, Feb). Latent membrane protein 1 and macrophage-derived TNFα synergistically activate and mobilize invadopodia to drive invasion of nasopharyngeal carcinoma. Journal of Pathology (7.2%, 7/90 PATHOLOGY), 10.1002/path.6036
5. Di Ngoc Kha Vo, Ha Phan Thanh Ho, Tung-Yi Lin, Jo-Ning Hung, Ya-Hui Chiu, Ming-Han Tsai*(2022, Sep).Ganoderma microsporum immunomodulatory protein acts as a multifunctional broadspectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusion. BIOMEDICINE & PHARMACOTHERAPY (5.3%, 19/3522 PHARMACOLOGY & PHARMACY), Vol 155, 113766. doi.org/10.1016/j.biopha.2022.113766. (*corresponding)
6. Jo-Ning Hung, Di Ngoc Kha Vo, Ha Phan Thanh Ho, Ming-Han Tsai* (2022, Aug). PEDOT:PSS in solution form exhibits a strong potential in inhibiting SARS-CoV-2 infection of the host cells by targeting viruses and also the host cells. BIOMACROMOLECULES (ACS publications)(4.4%, 3/57 CHEMISTRY, ORGANIC) 23(9),3535-3548. doi.org/10.1021/acs.biomac.2c00271. (*corresponding)
7. Hsin Yeh, Di Ngoc Kha Vo, Zhi-Hu Lin, Ha Phan Thanh Ho, Wei-Jyun Hua, Wei-Lun Qiu, Ming-Han Tsai*, Tung-Yi Lin* (2022, May). GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus. PHYTOMEDICINE(1%, 1/45 INTEGRATIVE & COMPLEMENTARY MEDICINE, IF 8.2), 103:154215. doi: 10.1016/j.phymed.2022.154215. (*corresponding)
8. Jen-Mei Chou, Jo-Ling Tsai, Jo-Ning Hung, I-Hua Chen, Szu-Ting Chen, MingHan Tsai* (2022, May). The ORF8 Protein of SARS-CoV-2 Modulates the Spike Protein and Its Implications in Viral Transmission. Frontiers in Microbiology, 19;13:883597. (*corresponding)
9. Zhe Li, Francesco Baccianti, Susanne Delecluse, Ming-Han Tsai, Anatoliy Shumilov, Xianliang Cheng, Sicong Ma, Ingrid Hoffmann, Remy Poirey, Henri-Jacques Delecluse (2021, Oct). The Epstein-Barr virus non-coding RNA EBER2 transactivates the UCHL1 deubiquitinase to accelerate cell growth. PNAS (10%, 14/135 MULTIDISCIPLINARY SCIENCES, IF 9.1), 118 (43) e2115508118
10. Hsieh, H.Y., Chang, R., Huang, Y.Y., Juan, P.H., Tahara, H., Lee, K.Y., Vo, D.N.K., Tsai, M.H., Wei, P.K., Sheen, H.J., Fan, Y.J.  , 2021. Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection. Biosensors and Bioelectronics (2.3%, 3/111 CHEMISTRY, ANALYTICAL, IF 10.5) 10.1016/j.bios.2021.113672
11. Susanne Delecluse, Remy Poirey, Martin Zeier, Paul Schnitzler, Uta Behrends, Ming-Han Tsai*, Henri-Jacques Delecluse*  2020.  Identification and cloning of a new Western Epstein-Barr Virus strain that efficiently replicates in primary B cells. J. Virol. DOI: 10.1128/JVI.01918-19. (*corresponding)
12. Zhe Li#, Ming-Han Tsai#, Anatoliy Shumilov, Francesco Baccianti, Sai Wah Tsao, Remy Poirey, R, Henr-Jacques Delecluse,(2019, Sep). A non-coding RNA from a carcinogenic virus induces an inflammatory response to promote virus production in infected cells. Nature Microbiology (2%, 4/163 MICROBIOLOGY, IF 19.2). doi: 10.1038/s41564-019-0546-y (#co-first author)
13. Delecluse, S., Tsai, M.H., Shumilov, A., Bencun, M., Arrow, S., Beshirova, A., Cottignies-Calamarte, A., Lasitschka, F., Bulut, OC., Münz, C., Zeier, M., Behrends, U., Delecluse, H.J.. 2019. Epstein-Barr virus induces expression of the LPAM-1 integrin in B cells in vitro and in vivo. J. Virol. 19;93(5). pii: e01618-18.
14. Tian, J.N., Yang, C.C., Chuang, C.K., Tsai, M.H., Wu, R.H., Chen, C.T., Yueh. A. 2019. A Dengue virus type 2 (DENV-2) NS4B-Interacting host factor, SERP1, reduces DENV-2 production by suppressing viral RNA replication. Viruses 11(9):787. doi: 10.3390/v11090787.
15. van Zyl, D.G.,Tsai, M.H., Shumilov, A., Schneidt, V., Poirey, R., Schlehe, B., Fluhr, H., Mautner, J., Delecluse, H.J.. 2018. Immunogenic particles with a broad antigenic spectrum stimulate cytolytic T cells and offer increased protection against EBV infection ex vivo and in mice. PLoS Pathogens 14(12):e1007464
16. Bencun, M., Klinke, O., Hotz-Wagenblatt, A., Klaus, S., Tsai, M.H., Poirey, R., Delecluse, H.J. 2018. Translational profiling of B cells infected with the Epstein-Barr virus reveals 5’ leader ribosome recruitment through upstream opening reading frames. Nucleic Acids Res. 6;46(6):2802-2819.
17. Gupta, S., Ylä-Anttila, P., Callegari, S., Tsai, M.H., Delecluse, H.J., Masucci, M.G. 2018. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathogens 14(1):e1006852.

A2. Up to 2017​

1. Shumilov, A.*, Tsai, M.H.*, (*co-first author, listed by last name alphabetically), Schlosser, Y., Kratz, A.S., Bernhardt, K., Fink, S., Mizani, T., Lin, X., Jauch, A., Mautner, J., et al. 2017. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nature Communications. doi:10.1038/ncomms14257.
2. Wu, R.H., Tsai, M.H., Tsai, K.N., Tian, J.N., Wu, J.S., Wu, S.Y., Chern, J.H., Chen, C.H., and Yueh, A. 2017. Mutagenesis of Dengue Virus Protein NS2A Revealed a Novel Domain Responsible for Virus-Induced Cytopathic Effect and Interactions Between NS2A and NS2B Transmembrane Segments. J. Virol doi: 10.1128/jvi.01836-16
3. Ma, S.D.*, Tsai, M.H.* (*co-first author, listed by last name alphabetically), Romero-Masters, J., Ranheim, E.A., Bristol, J.A., Delecluse, H.J., and Kenney, S.C. 2017. Epstein-Barr virus (EBV) can induce B cell lymphomas in a cord blood-humanized mouse model in the absence of LMP2A and LMP1 expression. J. Virol. doi: 10.1128/jvi.01928-16.
4. Bilger, A., Plowshay, J., Ma, S.D., Nawandar, D., Barlow, E.A., Romero-Masters, J.C., Bristol, J.A., Li, Z.,  Tsai, M.H., Delecluse, H.J., Kenny, S.C. 2017. Leflunomide/teriflunomide inhibit EBV-induced lymphoproliferative disease and lytic replication. Oncotarget. 8: 44266-33280
5. Fink, S., Tsai, M.H., Schnitzler, P., Zeier, M., Dreger, P., Wuchter, P., Bulut, O.C., Behrends, U., and Delecluse, H.J. 2017. The Epstein-Barr virus DNA load in the peripheral blood of transplant recipients does not accurately reflect the burden of infected cells. Transpl Int 30:57-67.
6. Tsai, M.H., Lin, X., Shumilov, A., Bernhardt, K., Feederle, R., Poirey, R., Kopp-Schneider, A., Pereira, B., Almeida, R., and Delecluse, H.J. 2016. The biological properties of different Epstein-Barr virus strains explain their association with various types of cancers. Oncotarget. 10.18632/oncotarget.14380
7. Bernhardt, K., Haar, J., Tsai, M.H., Poirey, R., Feederle, R., and Delecluse, H.J. 2016. A Viral microRNA Cluster Regulates the Expression of PTEN, p27 and of a bcl-2 Homolog. PLoS Pathogens 12:e1005405.
8. Wu, R.H., Tsai, M.H., Chao, D.Y., and Yueh, A. 2015. Scanning mutagenesis studies reveal a potential intramolecular interaction within the C-terminal half of dengue virus NS2A involved in viral RNA replication and virus assembly and secretion. J. Virol. 89:4281-4295.
9. Lin, XC*, Tsai, M.H.* (*co-first author, listed by last name alphabetically), Shumilov, A., Poirey, R., Bannert, H., Middeldorp, J.M., Feederle, R., and Delecluse, H.J. 2015. The Epstein-Barr Virus BART miRNA Cluster of the M81 Strain Modulates Multiple Functions in Primary B Cells. PLoS Pathogens 11:e1005344.
10. Hou, M.S., Huang, S.T., Tsai, M.H., Yen, C.C., Lai, Y.G., Liou, Y.H., Lin, C.K., and Liao, N.S. 2015. The interleukin-15 system suppresses T cell-mediated autoimmunity by regulating negative selection and nT(H)17 cell homeostasis in the thymus. J Autoimmun 56:118-129.
11. Feederle, R., Klinke, O., Kutikhin, A., Poirey, R., Tsai, M.H., and Delecluse, H.J. 2015. Epstein-Barr Virus: From the Detection of Sequence Polymorphisms to the Recognition of Viral Types. Curr Top Microbiol Immunol 390:119-148.
12. Pu, S.Y., Wu, R.H., Tsai, M.H., Yang, C.C., Chang, C.M., and Yueh, A. 2014. A novel approach to propagate flavivirus infectious cDNA clones in bacteria by introducing tandem repeat sequences upstream of virus genome. J Gen Virol 95:1493-1503.
13. Antsiferova, O., Muller, A., Ramer, P.C., Chijioke, O., Chatterjee, B., Raykova, A., Planas, R., Sospedra, M., Shumilov, A., Tsai, M.H., et al. 2014. Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. PLoS Pathogens 10:e1004333.
14. Yang, C.C., Tsai, M.H., Hu, H.S., Pu, S.Y., Wu, R.H., Wu, S.H., Lin, H.M., Song, J.S., Chao, Y.S., and Yueh, A. 2013. Characterization of an efficient dengue virus replicon for development of assays of discovery of small molecules against dengue virus. Antiviral Res 98:228-241.
15. Tsai, M.H., Raykova, A., Klinke, O., Bernhardt, K., Gartner, K., Leung, C.S., Geletneky, K., Sertel, S., Munz, C., Feederle, R., et al. 2013. Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas. Cell Rep 5:458-470. (Spotlight article in the review Frappier, L. 2013. A cancer-associated Epstein-Barr virus variant with epithelial tropism. Trends Microbiol 21:616-617)
16. Pu, S.Y., Wu, R.H., Yang, C.C., Jao, T.M., Tsai, M.H., Wang, J.C., Lin, H.M., Chao, Y.S., and Yueh, A. 2011. Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes. J. Virol. 85:2927-2941.
17. Lai, Y.G., Hou, M.S., Hsu, Y.W., Chang, C.L., Liou, Y.H., Tsai, M.H., Lee, F., and Liao, N.S. 2008. IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine. J Immunol 180:3757-3765.

專利與發明 :

1. 發明專利: 預防皰疹病毒感染人類細胞的方法
   METHOD FOR PREVENTING HUMAN CELL INFECTION BY HERPESVIRUSES
   美國US 12,018,055 B2 蔡明翰,林東毅 國立陽明交通大學

2. 發明專利: 一種包含聚3,4-乙烯二氧噻吩-聚苯乙烯磺酸鹽(PEDOT:PSS)之抗病毒組合物用於製備預防或治療冠狀病毒相關疾病之醫藥組合物以及其用於在活體外降低冠狀病毒感染率的用途
   中華民國I823333, 蔡明翰, 國立陽明交通大學
3. 發明專利:IMPROVED EBV VACCINEUS;
   WO; JP; CNUS11097003B2 Henri-Jacques Delecluse, Anatoliy Shumilov, Ming-Han Tsai, Deutsches Krebsforschungszentrum (DKFZ), Germany
4. 發明專利: IMMUNOMODUL ATORY PROTEIN FOR PREVENTION OF CORONAVIRUS INFECTIONUS;
   TWUS2023233632A1, Tung-Yi Lin, Ming-Han Tsai, National Yang Ming Chiao Tung University
5. 發明專利: METHODS OF PREVENTING OR TREATING CORONAVIRUS INFECTION REDUCING CORONAVIRUS RATE IN VITRO WITH ANTIVIRAL COMPOSITION COMPRISING POLY(3,4 ETHYLENEDIOXYTHIOPHENE):POLY(STYRENE SULFONATE)
   US; TW US20230321138A1
   Ming-Han Tsai, National Yang Ming Chiao Tung University​​

PRESENTATIONS

1. EBV and human diseases. Invited speaker in 86th Japanese Society of Hematology, Kyoto, Japan, Oct 2024
2. Pathogenic roles of EBV strains. Invited speaker at University of Nagoya, Japan, Oct 2024
3. The Epstein-Barr virus induces the expression of LPAM-1 in B-cells to access the gastrointestinal mucosa-associated lymphoid tissues. 18th International Symposium on EBV and associated diseases, Madison, Wisconsin, July 28-Aug 1, 2018.
4. The M81 Epstein-Barr Virus associated RNA 2 Enhances CXCL8 production and Spontaneous vius lytic replication in primary B lymphocytes. (Oral Presentation) Nasopharyngeal Carcinoma, Gordon Research Conference, Hong Kong, June 24-29, 2018.
5. The Epstein-Barr virus BART miRNA cluster of the nasopharyngeal carcinoma-associated M81 strain modulates lytic replication and cell growth in primary B cells. (Oral Presentation) 17th International Symposium on EBV and associated diseases, Zürich, Switzerland, Aug 8-12, 2016.
6. The Epstein-Barr virus BART miRNA cluster of the nasopharyngeal carcinoma-associated M81 strain modulates lytic replication and cell growth in primary B cells. Nasopharyngeal Carcinoma, Gordon Research Conference, Hong Kong, June 26-July 1, 2016.
7. Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas. EMBO meeting, Amsterdam, Sep 21-24 , 2013.

Current Research

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research
簡介: 我們對於癌症病毒，特別是EB病毒(EBV,台灣常稱鼻咽癌病毒)如何導致人類上皮細胞癌深感興趣。除了鼻咽癌，一種和EBV絕對相關的癌症，目前也發現EBV在胃癌、肺癌、乳癌、大腸癌、淋巴癌、胸腺癌等各類癌症，甚至多種自體免疫疾病，都可大量發現EBV感染的證據。很可惜EBV到底如何導致這些疾病仍不清楚。
我們實驗室具備全世界對此病毒研究之最先進技術，當前我們將主力放在此病毒對胃癌造成之機制。我們也同時研究一些新發現由此病毒導致之癌症種類及疾病。
同時，我們也嘗試改造這些致癌病毒使之為我們所用。我們有專門技術可以去改造大體積之DNA病毒並且成功將之送到宿主細胞內。期待我們能將這些”壞壞”的病毒轉化變成人類有用的工具。竭誠歡迎您加入我們並用研究病毒來改變這個世界!

詳細研究資訊請點選 https://wisewind1005.wixsite.com/tsaimh-ebv/research

實驗室成員

當前人員及先前成員：https://wisewind1005.wixsite.com/tsaimh-ebv/people

當前成員：
博士生：吳立萱(Emily), 武玉可怡(Zoe)
碩士生：張敬慈(Kei), 鄭氏越項(Jade), 裴竹薇(Vy), 鄭立晴(Sally), 阮玉明恒(Minh), 邱苠峰(Mike), 蔡瑀倢(Jessica)

實驗室照片